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Investigate the intracellular physiological conditions and triggers required to induce butanol-synthesizing enzymes in Clostridium acetobutylicum.

July 1, 1986Applied and Environmental MicrobiologyOpen Access

Intracellular Conditions Required for Initiation of Solvent Production by Clostridium acetobutylicum

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Authors

JTJoseph TerraccianoMerck & Co., Inc., Rahway, NJ, USA (United States)EKEva R. KashketBoston University

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Implication

Mechanistic study reveals critical intracellular organic acid thresholds initiate solventogenesis in Clostridium acetobutylicum, highlighting drivers of bacterial butanol fermentation.

Key Points

Investigate the intracellular physiological conditions and triggers required to induce butanol-synthesizing enzymes in Clostridium acetobutylicum.
Monitored internal and external pH, membrane potential, and proton motive force across growth phases in batch fermentations.
Evaluated the impact of initial 50 mM butyric acid supplementation and different buffer systems (phosphate versus acetate) on metabolic transition.
Assessed the induction kinetics of solvent-producing enzymes by tracking rates of butyrate conversion to butanol.
Internal pH remained at or above 5.5 during batch growth, while butanol accumulation dissipated the proton motive force predominantly by diminishing the transmembrane pH gradient.
Solventogenesis initiated when intracellular undissociated butyric acid reached 13 mM and total intracellular undissociated acids reached 40 to 45 mM.
The rate of butyrate-to-butanol conversion peaked at 50 mM butanol during the mid-solvent phase, after which de novo enzyme synthesis declined and ceased.

Cite This Study

Terracciano et al. (1986) studied this question.

synapsesocial.com/papers/6a7e06571ec832c82ccdbccb https://doi.org/10.1128/aem.52.1.86-91.1986

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Implication

Mechanistic study reveals critical intracellular organic acid thresholds initiate solventogenesis in Clostridium acetobutylicum, highlighting drivers of bacterial butanol fermentation.

Intracellular Conditions Required for Initiation of Solvent Production by Clostridium acetobutylicum

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Implication

Key Points

Cite This Study

Intracellular Conditions Required for Initiation of Solvent Production by Clostridium acetobutylicum

Discussion

Member takes

Implication

Key Points

Cite This Study

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