What question did this study set out to answer?

Investigate the molecular mechanism by which protein kinase C activation modulates fast transient potassium A-channels expressed from the Shaker H4 clone.

February 25, 1991FEBS LettersOpen Access

Modulation of a Shaker potassium A‐channel by protein kinase C activation

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Population

Xenopus oocytes expressing A-channels from the Shaker H4 clone

Design

Preclinical

Authors

OMOfira MoranTel Aviv University NDNathan DascalElectrophysiology ILIlana LotanTel Aviv University

Discussion

Member takes

Implication

Provides mechanistic insight into PKC regulation of A-type K+ currents; leaves open relevance to human cardiac electrophysiology.

Key Points

Investigate the molecular mechanism by which protein kinase C activation modulates fast transient potassium A-channels expressed from the Shaker H4 clone.
Expressed Shaker H4 clone A-type potassium channels in Xenopus oocytes.
Assessed electrophysiological properties before and after protein kinase C activation using two-electrode voltage-clamp recordings.
Protein kinase C activation exerts an inhibitory effect primarily by reducing the maximal conductance of the channels.
Channel modulation causes a secondary, small change in the voltage-dependence of activation and inactivation.

Structured PICO

Population

Xenopus oocytes expressing A-channels from the Shaker H4 clone

Intervention

Protein kinase C (PKC) activation

Outcome

Modulation of channel properties (maximal conductance, voltage-dependence of activation and inactivation)surrogate

PKC activation inhibits Shaker H4 A-channels expressed in Xenopus oocytes primarily by reducing maximal conductance.

Cite This Study

Moran et al. (1991) studied this question.

synapsesocial.com/papers/6a7e1403fc4f0e65bd3bfa81 https://doi.org/10.1016/0014-5793(91)80162-v

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Modulation of a Shaker potassium A‐channel by protein kinase C activation

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Modulation of a Shaker potassium A‐channel by protein kinase C activation

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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