December 16, 2015Cardiovascular ResearchOpen Access

Loss of plakoglobin immunoreactivity in intercalated discs in arrhythmogenic right ventricular cardiomyopathy: protein mislocalization versus epitope masking

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Population

Desmoglein 2-mutant mice with an ARVC-like phenotype and cardiac biopsies from humans with ARVC and non-ARVC…

Design

Preclinical

Authors

SKSebastian KantRWTH Aachen University CKClaudia A. KruscheRWTH Aachen University AGAnna GaertnerUniversity Hospitals of the Ruhr-University of Bochum

Discussion

Member takes

Implication

Questions plakoglobin immunostaining reliability for ARVC diagnosis; leaves open true protein changes and biomarker validity.

Structured PICO

Population

Desmoglein 2-mutant mice with an ARVC-like phenotype (n=6) and cardiac biopsies from humans with ARVC and non-ARVC heart disease (n=10)

Intervention

Immunofluorescence staining with multiple antibodies against different plakoglobin epitopes

Outcome

Plakoglobin immunoreactivity in intercalated discssurrogate

Reduced plakoglobin staining in ARVC is due to epitope masking rather than true protein mislocalization, which has implications for its use as a diagnostic biomarker.

Cite This Study

Kant et al. (2015) studied this question.

synapsesocial.com/papers/6a7e261297cd01de9cb1f16a https://doi.org/10.1093/cvr/cvv270

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Loss of plakoglobin immunoreactivity in intercalated discs in arrhythmogenic right ventricular cardiomyopathy: protein mislocalization versus epitope masking

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Loss of plakoglobin immunoreactivity in intercalated discs in arrhythmogenic right ventricular cardiomyopathy: protein mislocalization versus epitope masking

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider