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The amino acid sequences of four cyanogen bromide (CB) fragments from rabbit skeletal troponin-T have been determined. Fragment CB4 (55 residues) was documented by chymotryptic, thermolytic, tryptic, and 2-(2-nitrophenylsulfenyl)-3-methyl-3'-bromoindolenine peptides. A combination of automated sequence analysis, tryptic digestion, and penicillocarboxypeptidase-S treatment was used to assemble fragments CB5 (24 residues), CB7 (8 residues), and the COOH-terminal CB6 (21 residues).
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Pearlstone et al. (1977) studied this question.
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