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Develop and validate a molecular-level method to quantify condensed tannin monomers and adducts in complex environmental samples using acid depolymerization and capillary gas chromatography.

September 12, 2000Analytical Chemistry

Determination of Condensed Tannin Monomers in Environmental Samples by Capillary Gas Chromatography of Acid Depolymerization Extracts

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Authors

PHPeter J. HernesUniversity of California, Davis JHJohn I. HedgesPennsylvania State University

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Implication

Analytical study demonstrates precise gas chromatographic quantification of condensed tannins in leaf litter and soils, highlighting the analytical significance of tannin-mineral interactions.

Key Points

Develop and validate a molecular-level method to quantify condensed tannin monomers and adducts in complex environmental samples using acid depolymerization and capillary gas chromatography.
Optimized acid depolymerization and carbocation capture with phloroglucinol across variations in acid strength, reagent concentration, reaction time, and temperature.
Derivatized monomer and adduct products with trimethylsilyl groups, separated them via capillary gas chromatography, and quantified them using flame ionization detection against standards.
Tested the analytical method across environmental matrices, including leaf litter, soil, and sediment samples.
Achieved reproducible measurements in leaf litter with linear response across 2 orders of magnitude and a lower detection limit of approximately 100 ng condensed tannin.
Identified and successfully quantified triterpenoid compounds simultaneously within the same depolymerization extracts.
Determined that condensed tannin interactions with mineral phases in soils and sediments strongly influence both quantitative analytical recovery and environmental behavior.

Cite This Study

Hernes et al. (2000) studied this question.

synapsesocial.com/papers/6a7e4c2b3eabdb005bb29c99 https://doi.org/10.1021/ac991301y

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Implication

Analytical study demonstrates precise gas chromatographic quantification of condensed tannins in leaf litter and soils, highlighting the analytical significance of tannin-mineral interactions.

Determination of Condensed Tannin Monomers in Environmental Samples by Capillary Gas Chromatography of Acid Depolymerization Extracts

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Implication

Key Points

Cite This Study

Determination of Condensed Tannin Monomers in Environmental Samples by Capillary Gas Chromatography of Acid Depolymerization Extracts

Discussion

Member takes

Implication

Key Points

Cite This Study

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