What question did this study set out to answer?

To examine how activators of cyclic AMP-dependent protein kinase and protein kinase C affect the membrane properties and ionic currents of central and peripheral mouse neurons.

January 16, 1987Science

Forskolin and Phorbol Esters Reduce the Same Potassium Conductance of Mouse Neurons in Culture

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Population

Mouse dorsal root ganglion neurons and cerebral hemisphere neurons grown in primary dissociated cell culture

Design

Preclinical

Authors

DGDebra S. GregaRoche (United States)MWMary Ann WerzUniversity of Iowa RMRobert L. MacdonaldGoogle (United States)

Discussion

Member takes

Implication

Convergent second-messenger effects on neuronal K+ conductance in mice; leaves open relevance to cardiac ion-channel regulation.

Key Points

To examine how activators of cyclic AMP-dependent protein kinase and protein kinase C affect the membrane properties and ionic currents of central and peripheral mouse neurons.
Cultured primary dissociated mouse dorsal root ganglion neurons and cerebral hemisphere neurons in vitro.
Applied forskolin (to activate cyclic AMP-dependent protein kinase) and phorbol 12,13-dibutyrate (to activate protein kinase C).
Recorded resting membrane properties, action potentials, and membrane currents using the single-electrode voltage clamp technique.
Neither forskolin nor phorbol 12,13-dibutyrate altered resting membrane properties in central or peripheral neurons.
Both forskolin and phorbol 12,13-dibutyrate extended the duration of calcium-dependent action potentials in both cell populations.
Single-electrode voltage clamp analysis demonstrated that both agents decreased the same voltage-dependent potassium conductance.

Structured PICO

Population

Mouse dorsal root ganglion neurons and cerebral hemisphere neurons grown in primary dissociated cell culture

Intervention

Forskolin (FOR) and phorbol 12,13-dibutyrate (PDBu)

Outcome

Resting membrane properties, action potentials, and voltage-dependent potassium conductancesurrogate

Two independent second messenger systems (cAMP and phosphatidylinositol) may affect the same potassium conductance in mouse neurons.

Cite This Study

Grega et al. (1987) studied this question.

synapsesocial.com/papers/6a7e5400cc2a0d08ea856373 https://doi.org/10.1126/science.2432663

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Forskolin and Phorbol Esters Reduce the Same Potassium Conductance of Mouse Neurons in Culture

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Forskolin and Phorbol Esters Reduce the Same Potassium Conductance of Mouse Neurons in Culture

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider