What question did this study set out to answer?

To generate and characterize monoclonal antibodies against murine complement receptor type 1 (CR1) and define its cellular distribution across mouse hemopoietic cells.

May 1, 1988The Journal of Immunology

Monoclonal antibodies to mouse complement receptor type 1 (CR1). Their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative.

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Authors

TKTaroh KinoshitaThe University of Osaka JTJunji TakedaThe University of Osaka HKHaruo KozonoNational Institutes for Quantum Science and Technology

Discussion

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Implication

Laboratory study demonstrates the absence of complement receptor type 1 on mouse erythrocytes and platelets, suggesting an alternative mechanism for clearing immune complexes.

Key Points

To generate and characterize monoclonal antibodies against murine complement receptor type 1 (CR1) and define its cellular distribution across mouse hemopoietic cells.
Generated three monoclonal antibodies (8C12, 7G6, 7E9) and evaluated their target specificity using immunoprecipitation, rosette inhibition, and factor I cofactor activity assays.
Profiled CR1 expression across murine lymphoid, myeloid, erythroid, and platelet lineages via radiolabeled antibody binding and fluorescent flow cytometry.
Antibody 8C12 bound the ligand-binding domain of a 190-kDa protein, identifying CR1 on spleen B lymphocytes, peritoneal macrophages, and stimulated granulocytes, but not on T lymphocytes.
Mouse erythrocytes and platelets lacked CR1 expression across all antibody binding, immunoprecipitation, and functional blocking evaluations.
Platelet immune adherence to C3b-bearing cells occurred independently of CR1, revealing that mouse platelets utilize an unidentified C3b-binding factor.

Cite This Study

Kinoshita et al. (1988) studied this question.

synapsesocial.com/papers/6a7e66b691d72aebec32fad0 https://doi.org/10.4049/jimmunol.140.9.3066

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May 1, 1988The Journal of Immunology

Monoclonal antibodies to mouse complement receptor type 1 (CR1). Their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative.

View Full Paper

Ask AI

Bookmark

Authors

TKTaroh KinoshitaThe University of Osaka JTJunji TakedaThe University of Osaka HKHaruo KozonoNational Institutes for Quantum Science and Technology

Discussion

Member takes

Implication

Laboratory study demonstrates the absence of complement receptor type 1 on mouse erythrocytes and platelets, suggesting an alternative mechanism for clearing immune complexes.

Key Points

To generate and characterize monoclonal antibodies against murine complement receptor type 1 (CR1) and define its cellular distribution across mouse hemopoietic cells.
Generated three monoclonal antibodies (8C12, 7G6, 7E9) and evaluated their target specificity using immunoprecipitation, rosette inhibition, and factor I cofactor activity assays.
Profiled CR1 expression across murine lymphoid, myeloid, erythroid, and platelet lineages via radiolabeled antibody binding and fluorescent flow cytometry.
Antibody 8C12 bound the ligand-binding domain of a 190-kDa protein, identifying CR1 on spleen B lymphocytes, peritoneal macrophages, and stimulated granulocytes, but not on T lymphocytes.
Mouse erythrocytes and platelets lacked CR1 expression across all antibody binding, immunoprecipitation, and functional blocking evaluations.
Platelet immune adherence to C3b-bearing cells occurred independently of CR1, revealing that mouse platelets utilize an unidentified C3b-binding factor.

Cite This Study

Kinoshita et al. (1988) studied this question.

synapsesocial.com/papers/6a7e66b691d72aebec32fad0 https://doi.org/10.4049/jimmunol.140.9.3066

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