What question did this study set out to answer?

To identify and characterize temperature-sensitive mutations that selectively disrupt distinct stages of fin tissue regeneration.

December 1, 1995GeneticsOpen Access

Temperature-sensitive mutations that cause stage-specific defects in Zebrafish fin regeneration.

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Genetic screening reveals stage-specific regeneration failure in zebrafish, indicating distinct temporal controls govern tissue regrowth.

To identify and characterize temperature-sensitive mutations that selectively disrupt distinct stages of fin tissue regeneration.
Conducted a genetic screen in adult zebrafish to isolate temperature-sensitive mutants with impaired caudal fin regeneration.
Performed temperature-shift assays on isolated mutants, specifically reg5 and reg6, to define stage-specific critical periods during regeneration and development.
Identified seven temperature-sensitive mutations affecting regeneration: five causing complete regeneration failure, one conferring slow growth, and one forming dysmorphic bumps.
Temperature-shift experiments revealed that reg5 acts during early blastema formation, preventing blastema expansion and bone-forming cell differentiation.
The reg6 mutation acts later during regenerative outgrowth following bone differentiation, with both reg5 and reg6 also causing defects during embryonic or juvenile fin development.

Johnson et al. (1995) studied this question.

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