Scanning electron microscopy provides high-resolution 3-dimensional visualization of the intracellular architecture and connective tissue in hypertrophic cardiomyopathy.
Demonstrates SEM feasibility for 3D HCM tissue architecture in one case; hypothesis-generating and leaves open any clinical or research utility.
S canning electron microscopy is a useful modality to visualize the 3-dimensional structures of tissues at high resolution. In addition, processing the tissue samples can provide further architectural information: The removal of connective tissue around the cardiomyocytes and, conversely, the removal of nonfibrous tissue enable us to clearly observe the cardiomyocytes and connective tissue, respectively. Furthermore, backscattered electron emission after heavy-metal staining can provide higher-quality images of the intracellular architecture. We have previously reported the 3-dimensional structure of the human left ventricular myocardium. 2 Here, we performed an electron microscopic examination of a myectomy specimen from a patient with hypertrophic cardiomyopathy using a Hitachi S-800 scanning electron microscope (Hitachi, Ltd, Tokyo, Japan). In this patient, asymmetrical hypertrophy of the left ventricular wall (interventricular septum, 19 mm; anterolateral wall, 14 mm) and a pressure gradient
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Kanzaki et al. (2012) studied this question.
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