Local aldosterone synthesis in the large intestine of mouse: An ex vivo incubation study

Figures

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  Figure 1.. Aldosterone (Aldo) concentration relative to the total protein amount in non-incubated intestinal samples of duodenum, jejunum and ileum of mice fed with a normal diet. Data presented as mean ± SEM; duodenum and jejunum, n = 6; ileum, n = 5, ***P < 0.005; between-group comparisons were undertaken using one-way analysis of variance followed by the Least Significant Difference test.

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  Figure 2.. Liver receptor homolog-1 (LRH-1) concentration relative to the total protein amount in non-incubated intestinal tissue samples from three groups of mice fed diets with different concentrations of sodium (Na) during a 14-day study. Data presented as mean ± SEM; colon and caecum, n = 6; **P < 0.01, ***P < 0.005; between-group comparisons were undertaken using one-way analysis of variance followed by the Least Significant Difference test.

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  Figure 3.. Corticosterone release into the incubation fluid from the colons of three groups of mice fed diets with different concentrations of sodium (Na) during a 14-day study: tissues incubated without (Control) or with either angiotensin II (Ang II; 1 μM) or dibutyryl-cAMP (DBA; 0.1 mM). The corticosterone concentrations in the incubation fluid were normalized relative to the total protein amount in incubated tissue samples. Data presented as mean ± SEM; n = 5–6/group; *P < 0.05; between-group comparisons were undertaken using one-way analysis of variance followed by the Least Significant Difference test.

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  Figure 4.. Aldosterone synthase (CYP11B2) protein levels were measured using enzyme-linked immunosorbent assay and normalized relative to the total protein amount in the incubated colon and caecum tissue from three groups of mice fed diets with different concentrations of sodium (Na) during a 14-day study: tissues incubated without (Control) or with either angiotensin II (Ang II; 1 μM) or dibutyryl-cAMP (DBA; 0.1 mM). Data presented as mean ± SEM; n = 5–6/group; ***P < 0.005; between-group comparisons were undertaken using one-way analysis of variance followed by the Least Significant Difference test.

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  Figure 5.. Aldosterone synthase (CYP11B2) protein levels were measured using Western blot analysis in the incubated colon tissue from three groups of mice fed diets with different concentrations of sodium (Na) during a 14-day study: tissues incubated without (Control) or with either angiotensin II (Ang II; 1 μM) or dibutyryl-cAMP (DBA; 0.1 mM). (a) Bands of CYP11B2 (green) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (red) from the colon. GAPDH was utilized as a loading control. (b) Effects of Ang II and DBA in colon when the control group columns without stimulants were set as 1. Data presented as mean ± SEM; n = 5–6/group; *P < 0.05, **P < 0.01; between-group comparisons were undertaken using one-way analysis of variance followed by the Least Significant Difference test. The colour version of this figure is available at: http://imr.sagepub.com.

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  Figure 6.. Aldosterone synthase (CYP11B2) protein levels were measured using Western blot analysis in the incubated caecum tissue from three groups of mice fed diets with different concentrations of sodium (Na) during a 14-day study: tissues incubated without (Control) or with either angiotensin II (Ang II; 1 μM) or dibutyryl-cAMP (DBA; 0.1 mM). (a) Bands of CYP11B2 (green) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (red) from the caecum. GAPDH was utilized as a loading control. (b) Effects of Ang II and DBA in caecum when the control group columns without stimulants were set as 1. Data presented as mean ± SEM; n = 5–6/group; no significant between-group differences. The colour version of this figure is available at: http://imr.sagepub.com.

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  Figure 7.. Aldosterone synthase (CYP11B2) protein levels were measured using Western blot analysis in the non-incubated adrenal glands from three groups of mice fed diets with different concentrations of sodium (Na) during a 14-day study. Bands of CYP11B2 (green) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (red) from the adrenal glands. GAPDH was utilized as a loading control. The normal diet control group column was set as 1. The adrenal glands from six mice from each group were pooled as one single sample (n = 1). The colour version of this figure is available at: http://imr.sagepub.com.

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  Figure 8.. Aldosterone synthase (CYP11B2) protein levels were measured using enzyme-linked immunosorbent assay and normalized relative to the total protein amount in the incubated non-stimulated colon and caecum tissue from three groups of mice fed diets with different concentrations of sodium (Na) during a 14-day study. Data presented as mean ± SEM; n = 5–6/group; ***P < 0.005; between-group comparisons were undertaken using one-way analysis of variance followed by the Least Significant Difference test.

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  Figure 9.. Aldosterone levels released into the incubation fluid were measured using enzyme-linked immunosorbent assay and normalized relative to the total protein amount in the incubated colon and caecum tissue from three groups of mice fed diets with different concentrations of sodium (Na) during a 14-day study: tissues incubated without (Control) or with either angiotensin II (Ang II; 1 μM) or dibutyryl-cAMP (DBA; 0.1 mM). Data presented as mean ± SEM; n = 8–10/control group, n = 4–6/Ang II and DBA groups; *P < 0.05; *** P < 0.005; between-group comparisons were undertaken using one-way analysis of variance followed by the Least Significant Difference test.

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  Figure 10.. Aldosterone levels released into the incubation fluid were measured using enzyme-linked immunosorbent assay and normalized relative to the total protein amount in the incubated non-stimulated colon and caecum tissues from three groups of mice fed diets with different concentrations of sodium (Na) during a 14-day study. Data presented as mean ± SEM; n = 8–10/group; *P < 0.05; **P < 0.01; ***P < 0.005; between-group comparisons were undertaken using one-way analysis of variance followed by the Least Significant Difference test.