Dual peroxidase and colloidal gold-labeling study of angiotensin converting enzyme and angiotensin-like immunoreactivity in the rat subfornical organ
Population
Adult rats
Design
Preclinical
Authors
Discussion
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Implication
Highlights potential local angiotensin II generation in rodent SFO; leaves open functional and translational relevance to human cardiovascular regulation.
Structured PICO
P
Population
Adult rats
I
Intervention
Immunocytochemical labeling using peroxidase-antiperoxidase (PAP) or combined PAP and immunogold methods
O
Outcome
Cellular localization and relationships of angiotensin converting enzyme (ACE) and angiotensin-like immunoreactivity (AGLI) in the subfornical organ (SFO)
This study provides ultrastructural evidence that ACE is localized on cell membranes and organelles in the rat subfornical organ, positioning it to interact with angiotensin II precursors.
Limitations
- Limited penetration of the 10 nm gold particles
Cite This Study
Pickel et al. (1986) studied this question.