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A rapid semimicro assay for lymphocyte activation in man is described. It is based upon the stimulation of protein synthesis (SPS) in peripheral blood lymphocytes cultured in serum-free medium with soluble antigens, phytomitogens or allogeneic lymphocytes. Cellular response to PHA is discernible in 4 hr and response to antigen in 16 to 24 hr. Stimulation in mixed lymphocyte cultures can be detected as early as 8 to 10 hr and invariably by 42 hr. The assay permits detection of lymphocyte activation earlier and with greater precision than the widely employed thymidine incorporation blast transformation system.
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Adkinson et al. (1974) studied this question.
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