Epidemical and etiological study on hand, foot and mouth disease following EV-A71 vaccination in Xiangyang, China

Figure 1. Monthly distribution of total and severe HFMD cases Total number of HFMD cases caused by EV-A71, other HEVs and non-HEV (HEV negative) were 4415 and were confirmed using Real Time PCR Kits, specific EV-A71 and pan-HEVs.

Figure 2. Proportion of the untypeable and typeable enteroviruses identified in HFMD patients. The total numbers of 3703 specimens were used for serotyping with combination of real time RT-PCR, conventional RT-PCR and cell cultures.

Figure 3. Proportion of the confirmed enterovirus serotypes identified in HFMD patients. The total case numbers of confirmed serotypes were 3201 performed with combination of Real Time RT-PCR, conventional RT-PCR and cell cultures.

Figure 4. Monthly distribution of HFMD cases caused by the six major serotypes. A total number of HFMD cases caused by the six major serotypes is 3130, confirmed with combination of Real Time RT-PCR, conventional RT-PCR and cell cultures.

Figure 5. Phylogenetic dendrograms based on the partial VP1 nucleotide sequences of EV-A71 (a), CV-A16 (b), CV-A6 (c), CV-A5 (d), CV-A10 (e), CV-A2 (f). The dendrograms were constructed using the neighbor-joining method based on the alignment of the partial VP1-region sequences of Xiangyang strains and other strains downloaded from GenBank. Bootstrap values (%) for 1000 replicates were calculated, and only values > 70% are shown at the nodes. Red dots indicate Xiangyang strains, and black square indicate prototype strains.