January 1, 1994Journal of Biological ChemistryOpen Access

Secretion from rat basophilic RBL-2H3 cells is associated with diphosphorylation of myosin light chains by myosin light chain kinase as well as phosphorylation by protein kinase C.

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Population

Rat basophilic RBL-2H3 cells

Comparison

Stimulation with antigen or combination of… vs Unstimulated cells or cells treated with phorbol…

Design

Preclinical

Authors

OCOksoon Hong ChoiJohns Hopkins University RARobert AdelsteinHeart Failure / Cardiomyopathy MBMichael A. BeavenUniversity of Hawaiʻi at Mānoa

Discussion

Member takes

Implication

Does not inform clinical decisions; leaves open dual kinase targeting for secretion modulation pending in vivo validation.

Structured PICO

Population

Rat basophilic RBL-2H3 cells

Intervention

Stimulation with antigen or combination of A23187 (50 nM) and phorbol 12-myristate 13-acetate (20 nM)

Comparator

Unstimulated cells or cells treated with phorbol ester alone

Outcome

Phosphorylation of myosin light chains and rate/extent of degranulationsurrogate

Secretion from RBL-2H3 cells requires both diphosphorylation of myosin light chains by myosin light chain kinase and phosphorylation by protein kinase C.

Cite This Study

Choi et al. (1994) studied this question.

synapsesocial.com/papers/6a7ea575cb17bc2d1d0d242e https://doi.org/10.1016/s0021-9258(17)42382-9

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Secretion from rat basophilic RBL-2H3 cells is associated with diphosphorylation of myosin light chains by myosin light chain kinase as well as phosphorylation by protein kinase C.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Secretion from rat basophilic RBL-2H3 cells is associated with diphosphorylation of myosin light chains by myosin light chain kinase as well as phosphorylation by protein kinase C.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider