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September 1, 1985AJP Cell Physiology

A radioimmunoblotting method for measuring myosin light chain phosphorylation levels in smooth muscle

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Population

Bovine aortic smooth muscle and intact uterine smooth muscle

Comparison

Radioimmunoblotting method using antiserum to… vs Direct assay for phosphate, scanning of…

Design

Preclinical

Authors

DHDavid R. HathawayNational Heart Lung and Blood InstituteJHJoe R. HaeberleUniversity of Vermont

Discussion

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Implication

Validates novel radioimmunoblot for myosin light chain phosphorylation in animal smooth muscle; leaves open translation to human studies.

Structured PICO

P
Population
Bovine aortic smooth muscle and intact uterine smooth muscle
I
Intervention
Radioimmunoblotting method using antiserum to 20,000-Da light chains and 125I-protein A
C
Comparator
Direct assay for phosphate, scanning of Coomassie blue-stained gels, and two-dimensional electrophoretic method
O
Outcome
Measurement of molar stoichiometry of myosin light chain phosphorylationsurrogate

A novel radioimmunoblotting method accurately measures myosin light chain phosphorylation levels in intact smooth muscle, comparable to existing electrophoretic and direct phosphate assays.

Cite This Study

Hathaway et al. (1985) studied this question.

synapsesocial.com/papers/6a7ea575cb17bc2d1d0d2430https://doi.org/10.1152/ajpcell.1985.249.3.c345

Topics

Oxytocin
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1An electrophoretic study of the low-molecular-weight components of myosin1970 · 711 citations
  2. 2Ca2+ Dependent Phosphorylation of Bovine Aortic Actomyosin1978 · 58 citations
  3. 3PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT1951 · 318,705 citations
  4. 4High resolution two-dimensional electrophoresis of proteins.1975 · 19,371 citations