An immunomagnetic capture and real-time RT-PCR method can rapidly and quantitatively detect low levels of Hepatitis A virus in produce rinses.
Supports sensitive HAV detection in produce; leaves open validation for routine food safety use.
In this study, an immunomagnetic capture method and a real-time reverse transcription-PCR assay were used to quantify hepatitis A virus (HAV) in green onion and strawberry rinses. This combined protocol detected as low as 0.5 PFU HAV in produce rinses and concentrated HAV levels up to 20-fold.
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Shan et al. (2005) studied this question.
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