What question did this study set out to answer?

To establish an automated immunohistochemical protocol for simultaneous visualization and quantification of cell proliferation or apoptosis within glutathione S-transferase-positive preneoplastic hepatic foci in rats.

September 1, 1997Journal of Histochemistry & CytochemistryOpen Access

Automated Double Labeling of Proliferation and Apoptosis in Glutathione S-transferase-positive Hepatocytes in Rats

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Authors

BSBrian ShortResearch Triangle Park Foundation DZDawn ZimmermanSmithsonian Institution LSLester W. SchwartzGlaxoSmithKline (United States)

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Implication

Laboratory study demonstrates automated dual labeling of proliferation and apoptosis in rat liver foci, highlighting a rapid approach to evaluate tissue growth kinetics.

Key Points

To establish an automated immunohistochemical protocol for simultaneous visualization and quantification of cell proliferation or apoptosis within glutathione S-transferase-positive preneoplastic hepatic foci in rats.
Formalin-fixed, paraffin-embedded rat liver sections were processed on the TechMate Automated Staining System using a double-label avidin-biotin-immunoperoxidase assay completed within 10 hours.
Steam heat-induced epitope retrieval and proteolytic digestion were applied prior to chromogenic detection using nickel-enhanced diaminobenzidine (for BrdU and TUNEL) and VIP substrate (for GSTP).
Automated processing yielded distinct dual staining, presenting brown-black BrdU-positive nuclei or TUNEL-positive apoptotic bodies inside purple GSTP-positive hepatocytes.
The rapid automated procedure eliminated manual staining variability and allowed reliable quantification of cellular proliferation and cell death kinetics in altered liver foci.

Cite This Study

Short et al. (1997) studied this question.

synapsesocial.com/papers/6a7eb43c366dd125a6c300ee https://doi.org/10.1177/002215549704500913

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Implication

Laboratory study demonstrates automated dual labeling of proliferation and apoptosis in rat liver foci, highlighting a rapid approach to evaluate tissue growth kinetics.

Automated Double Labeling of Proliferation and Apoptosis in Glutathione S-transferase-positive Hepatocytes in Rats

Discussion

Member takes

Implication

Key Points

Cite This Study

Automated Double Labeling of Proliferation and Apoptosis in Glutathione S-transferase-positive Hepatocytes in Rats

Discussion

Member takes

Implication

Key Points

Cite This Study

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