Identification of potential crucial cuproptosis-related genes in myocardial ischemia-reperfusion injury through the bioinformatic analysis

Figures

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  Fig. 1. DEG levels in the myocardial tissue of the ischemia-reperfusion injury mice. Analyzing the microarray datasets, GSE61592 showed the differently expressed genes in the myocardial tissue of the ischemia-reperfusion injury mice as heat map (A) and volcano map (B).

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  Fig. 2. Expressions of cuproptosis related genes. (A) The cuproptosis-related gene expressions in the MI/RI are shown as heat maps. (B) The association of the cuproptosis related genes. (C) The PPI network of the cuproptosis related genes.

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  Fig. 3. Dlat, Pdhb, and Pdhα participated in the MI/RI. (A) The Venn Diagram of the differently expressed genes in MI/RI and cuproptosis related genes. (B) The Venn Diagram of the Dlat, Pdhb, and Pdhα-related top 50 proteins. (C) The PPI network of the 27 proteins commonly associated with Dlat, Pdhb, and Pdhα. (D) KEGG analysis of the 27 proteins commonly associated with Dlat, Pdhb, and Pdhα.

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  Fig. 4. Dlat, Pdhb, and Pdhα1 might serve as prognosis and diagnosis biomarkers of MI/RI. Analyzing the microarray datasets GSE61592, (A) the Dlat, Pdhb, and Pdhα1 expressions in the myocardial tissue of the ischemia-reperfusion injury mice was obtained. (B) The AUC of Dlat, Pdhb, and Pdhα1 in myocardial tissue of the ischemia-reperfusion injury mice was shown (*** p < 0.001).

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  Fig. 5. Pdhb was demonstrated to be a diagnosis biomarker of MI/RI. (A) Through analyzing the microarray datasets GSE83472, the Dlat, Pdhb, and Pdhα1 expressions in the myocardial tissue of the ischemia-reperfusion injury mice were obtained. (B) The Dlat, Pdhb, and Pdhα1 expressions in the myocardial tissue of the ischemia-reperfusion injury mice were detected by qPCR. (C) Receiver operating characteristics curve analysis was conducted to predict the diagnostic value of Pdhb, Dlat, and Pdhα1 (* p < 0.05, *** p < 0.001).

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  Fig. 6. The expression and AUC value of Dlat, Pdhb, and Pdhα1 in MI/RI patients. (A) The Dlat, Pdhb, and Pdhα1 expressions in the serum samples of the ischemia-reperfusion injury patients were detected by qPCR. (B) Receiver operating characteristics curve analysis was conducted to predict the diagnostic value of Pdhb, Dlat, and Pdhα1 (** p < 0.01, *** p < 0.001).