What question did this study set out to answer?

To determine whether cell-cell and cell-substrate interactions between extraembryonic ectoderm and visceral endoderm direct parietal endoderm differentiation in post-implantation mouse embryos.

April 1, 1981Development

Cell interactions and endoderm differentiation in cultured mouse embryos

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To determine whether cell-cell and cell-substrate interactions between extraembryonic ectoderm and visceral endoderm direct parietal endoderm differentiation in post-implantation mouse embryos.
Dissected 6.5-day mouse egg cylinders into embryonic and extraembryonic halves and cultured them in suspension in vitro for up to 7 days.
Cultured isolated visceral extraembryonic endoderm cells and intact egg cylinders denuded of visceral endoderm to track cell recolonization and phenotype changes on the extraembryonic ectoderm.
Extraembryonic fragments formed parietal endoderm after 4 days in culture, assembling a thick basement membrane and synthesizing high levels of laminin and Type IV procollagen.
Phenotypic transition did not occur in isolated visceral endoderm or embryonic fragments, whereas visceral endoderm recolonizing denuded extraembryonic ectoderm successfully adopted parietal characteristics.

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Implication

In vitro study reveals visceral endoderm conversion to parietal endoderm mediated by extraembryonic ectoderm contact in mouse embryos, indicating local tissue interactions drive cell fate.

Cite This Study

Hogan et al. (1981) studied this question.

synapsesocial.com/papers/6a7eb7cb6f6aff5b979a133d https://doi.org/10.1242/dev.62.1.379

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Implication

In vitro study reveals visceral endoderm conversion to parietal endoderm mediated by extraembryonic ectoderm contact in mouse embryos, indicating local tissue interactions drive cell fate.

Cell interactions and endoderm differentiation in cultured mouse embryos

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Discussion

Implication

Cite This Study

Cell interactions and endoderm differentiation in cultured mouse embryos

Key Points

Discussion

Implication

Cite This Study

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