What question did this study set out to answer?

To develop and validate a cost-effective, environmentally sustainable, and stability-indicating RP-HPLC method for the simultaneous quantification of silodosin and tadalafil in the presence of degradation products.

August 14, 2026Discover Chemistry.Open Access

Green stability indicating RP- HPLC method for simultaneous estimation of Silodosin and Tadalafil development validation and comparative evaluation using one way ANOVA

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Authors

YRYukti RanaHindu College of Pharmacy DMDr. Drashti MandaleHindu College of Pharmacy SUSiddik UgharatdarHindu College of Pharmacy

Discussion

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Implication

Method validation study demonstrates precise, green RP-HPLC quantification of silodosin and tadalafil in synthetic mixtures, highlighting its utility for routine quality control.

Key Points

To develop and validate a cost-effective, environmentally sustainable, and stability-indicating RP-HPLC method for the simultaneous quantification of silodosin and tadalafil in the presence of degradation products.
Chromatographic separation was performed on an Inertsil C18 column using an isocratic mobile phase of phosphate buffer and methanol (34:66, v/v) at 1.0 mL/min with UV detection at 220 nm.
Method validation followed ICH Q2(R2) guidelines, incorporating forced degradation testing, greenness assessments (GAPI, AGREE, AGREEprep, BAGI), and one-way ANOVA comparisons against degradation conditions and a reported method.
Silodosin and tadalafil eluted at 3.810 and 10.165 min, displaying linearity across 40–120 µg/mL and 25–75 µg/mL, respectively (r = 0.9983 for both), with recoveries of 99.34–100.98% and 98.51–99.78% and precision %RSD < 2%.
Forced degradation products were completely resolved from intact active ingredients, and one-way ANOVA confirmed no statistically significant variation across stress conditions (p > 0.05).

Cite This Study

Rana et al. (2026) studied this question.

synapsesocial.com/papers/6a7ec6c6b70b84ec8b912e4c https://doi.org/10.1007/s44371-026-00849-5

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Implication

Method validation study demonstrates precise, green RP-HPLC quantification of silodosin and tadalafil in synthetic mixtures, highlighting its utility for routine quality control.

Green stability indicating RP- HPLC method for simultaneous estimation of Silodosin and Tadalafil development validation and comparative evaluation using one way ANOVA

Discussion

Member takes

Implication

Key Points

Cite This Study

Green stability indicating RP- HPLC method for simultaneous estimation of Silodosin and Tadalafil development validation and comparative evaluation using one way ANOVA

Discussion

Member takes

Implication

Key Points

Cite This Study

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