What question did this study set out to answer?

To clone and functionally characterize the novel NAR2-family protein SaNAR2.2 and assess its interaction with high-affinity nitrate transporters in the halophyte Suaeda altissima.

August 14, 2026MembranesOpen Access

SaNAR2.2, A Novel Member of the NAR2 Family from the Euhalophyte Suaeda altissima: Molecular Cloning and Characterization

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To clone and functionally characterize the novel NAR2-family protein SaNAR2.2 and assess its interaction with high-affinity nitrate transporters in the halophyte Suaeda altissima.
Cloned the SaNAR2.2 coding sequence from Suaeda altissima and performed phylogenetic, topological, and subcellular localization predictions.
Evaluated protein-protein interactions between SaNAR2.2 and high-affinity nitrate transporters (SaNRT2.1 and SaNRT2.5) via bimolecular fluorescence complementation (BiFC) in Nicotiana benthamiana and a yeast mating-based split-ubiquitin system (mbSUS).
Measured tissue-specific expression and transcript level changes under low nitrate conditions combined with salt stress up to 0.5 M NaCl.
SaNAR2.2 encodes a predicted 196-amino acid (21.7 kDa) plasma membrane protein grouping within the NAR2.2 subfamily clade of Amaranthaceae.
BiFC and mbSUS assays confirmed direct physical interaction of SaNAR2.2 with partner transporters SaNRT2.1 and SaNRT2.5.
SaNAR2.2 expresses predominantly in roots and shows significant transcript upregulation during nitrate deficiency coupled with high salinity (up to 0.5 M NaCl).

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Implication

Molecular characterization reveals SaNAR2.2 interacts with NRT2 transporters in Suaeda altissima roots, highlighting its role in high-affinity nitrate uptake under extreme salinity.

Cite This Study

Khramov et al. (2026) studied this question.

synapsesocial.com/papers/6a7ec71db70b84ec8b913317 https://doi.org/10.3390/membranes16080267

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Implication

Molecular characterization reveals SaNAR2.2 interacts with NRT2 transporters in Suaeda altissima roots, highlighting its role in high-affinity nitrate uptake under extreme salinity.

SaNAR2.2, A Novel Member of the NAR2 Family from the Euhalophyte Suaeda altissima: Molecular Cloning and Characterization

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Discussion

Implication

Cite This Study

SaNAR2.2, A Novel Member of the NAR2 Family from the Euhalophyte Suaeda altissima: Molecular Cloning and Characterization

Key Points

Discussion

Implication

Cite This Study

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