What question did this study set out to answer?

To compare the performance of multiparameter flow cytometry (MFC) and droplet digital polymerase chain reaction (ddPCR) for measurable residual disease (MRD) monitoring in paediatric fusion gene-positive acute myeloid leukaemia.

August 14, 2026British Journal of Haematology

Multiparameter flow cytometry versus droplet digital polymerase chain reaction for monitoring measurable residual disease in paediatric fusion gene‐positive acute myeloid leukaemia

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To compare the performance of multiparameter flow cytometry (MFC) and droplet digital polymerase chain reaction (ddPCR) for measurable residual disease (MRD) monitoring in paediatric fusion gene-positive acute myeloid leukaemia.
Assessed MRD levels in N=116 paediatric patients with acute myeloid leukaemia across three post-treatment time points: end of induction 1 (EOI-1), end of induction 2 (EOI-2), and end of consolidation 1 (EOC-1).
Compared the prognostic value of MFC and ddPCR for predicting 5-year event-free survival (EFS) and evaluated performance across genetic alteration subgroups.
At EOI-1, MFC-MRD ≥1% was associated with a 5-year EFS of 50.2 ± 19.8% vs. 79.4 ± 6.4% for MRD <1% (p = 0.003), whereas ddPCR rates were 41.0 ± 23.8% vs. 91.7 ± 4.5% (p < 0.001).
The prognostic utility of MFC diminished by EOC-1, while ddPCR maintained significant prognostic discrimination across all time points and within all genetic subgroups.
Dual-positive MRD detection using both MFC and ddPCR identified a subset of patients with the lowest survival rates at EOI-1, EOI-2, and EOC-1.

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Implication

Cohort study reveals digital PCR offers superior sensitivity over flow cytometry in paediatric acute myeloid leukaemia, indicating that combining both methods improves risk stratification.

Cite This Study

Liu et al. (2026) studied this question.

synapsesocial.com/papers/6a7ec754b70b84ec8b913944 https://doi.org/10.1111/bjh.70714

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Implication

Cohort study reveals digital PCR offers superior sensitivity over flow cytometry in paediatric acute myeloid leukaemia, indicating that combining both methods improves risk stratification.

Multiparameter flow cytometry versus droplet digital polymerase chain reaction for monitoring measurable residual disease in paediatric fusion gene‐positive acute myeloid leukaemia

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Discussion

Implication

Cite This Study

Multiparameter flow cytometry versus droplet digital polymerase chain reaction for monitoring measurable residual disease in paediatric fusion gene‐positive acute myeloid leukaemia

Key Points

Discussion

Implication

Cite This Study

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