February 22, 2000Biochemistry

Role of the Structural Domain of Troponin C in Muscle Regulation: NMR Studies of Ca2+ Binding and Subsequent Interactions with Regions 1−40 and 96−115 of Troponin I

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Population

Isolated C-domain of skeletal troponin C (CTnC) and synthetic TnI peptides [TnI1-40 (or Rp40) and TnI96-115]

Design

Preclinical

Authors

PMPascal MercierWestern University MLMonica X. LiUniversity of Alberta BSBrian D. SykesHeart Failure / Cardiomyopathy

Discussion

Member takes

Implication

No immediate clinical impact; extends in vitro TnC-TnI models but leaves physiological relevance open.

Structured PICO

Population

Isolated C-domain of skeletal troponin C (CTnC) and synthetic TnI peptides [TnI1-40 (or Rp40) and TnI96-115]

Intervention

Calcium binding and peptide titration evaluated by 2D-1H,15N-HSQC NMR spectroscopy

Outcome

Stoichiometry, affinity, and mechanisms for binding of Ca2+ and TnI peptides to CTnCsurrogate

The N-terminal region of TnI binds to the structural domain of TnC with higher affinity than the inhibitory region, suggesting additional interactions are required for the inhibitory region to successfully compete during muscle regulation.

Cite This Study

Mercier et al. (2000) studied this question.

synapsesocial.com/papers/6a7ec891ec6d7e203fcda733 https://doi.org/10.1021/bi992579n

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Role of the Structural Domain of Troponin C in Muscle Regulation: NMR Studies of Ca2+ Binding and Subsequent Interactions with Regions 1−40 and 96−115 of Troponin I

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Role of the Structural Domain of Troponin C in Muscle Regulation: NMR Studies of Ca2+ Binding and Subsequent Interactions with Regions 1−40 and 96−115 of Troponin I

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider