What question did this study set out to answer?

Evaluate amplified ribosomal DNA restriction analysis of 16S rRNA genes as a rapid diagnostic method to differentiate the 18 genomic species within the genus Acinetobacter.

January 1, 1995Journal of Clinical MicrobiologyOpen Access

Identification of Acinetobacter genomic species by amplified ribosomal DNA restriction analysis

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Authors

MVMario VaneechoutteGhent University LDLenie DijkshoornLeiden University Medical Center ITIngela TjernbergMalmö University

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Implication

Laboratory evaluation demonstrates differentiation of Acinetobacter genomic species using ribosomal DNA restriction analysis, indicating a reliable tool for clinical and ecological tracking.

Key Points

Evaluate amplified ribosomal DNA restriction analysis of 16S rRNA genes as a rapid diagnostic method to differentiate the 18 genomic species within the genus Acinetobacter.
Tested 53 field and reference strains representing seven named species and 18 genomic species of Acinetobacter.
Amplified 16S rRNA genes enzymatically and digested them with restriction enzymes AluI, CfoI, MboI, RsaI, and MspI, followed by secondary enzyme analysis and four phenotypic confirmation tests.
Primary restriction digestion successfully differentiated 12 of the 18 genomic species, cleanly separating closely related pathogens including DNA groups 1 (A. calcoaceticus), 2 (A. baumannii), 3, and 13.
Three species pairs (groups 4 and 7, 5 and 17, and 10 and 11) shared identical restriction profiles across all tested enzymes but were differentiated using phenotypic tests for hemolysis, growth at 37°C, acid production from glucose, and gelatin hydrolysis.

Cite This Study

Vaneechoutte et al. (1995) studied this question.

synapsesocial.com/papers/6a7ee9eb084666b5350d3140 https://doi.org/10.1128/jcm.33.1.11-15.1995

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Implication

Laboratory evaluation demonstrates differentiation of Acinetobacter genomic species using ribosomal DNA restriction analysis, indicating a reliable tool for clinical and ecological tracking.

Identification of Acinetobacter genomic species by amplified ribosomal DNA restriction analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

Identification of Acinetobacter genomic species by amplified ribosomal DNA restriction analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

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