GFP for in vivo imaging of subcellular structures in plant cells

A novel marker, green fluorescent protein (GFP), has initiated a new era in cell biology because it permits real-time observations of dynamic changes in living cells and organisms. GFP-tagged fusion proteins are used to study gene expression, subcellular localization, and trafficking of proteins and organelles in vivo. GFP fusions have also revealed novel subcellular structures and provided new insights into compartmentation and protein exchange between compartments.