Site‐Specific and Stoichiometric Modification of Antibodies by Bacterial Transglutaminase

Spot on: Bacterial transglutaminase enables the site-specific modification of Gln side chains of tumor-targeting antibodies with various probes containing lysine or lysine surrogates. The method yields completely homogeneous immunoconjugates with a defined stoichiometry. In comparative in vivo studies with xenografted mice the pharmacological profiles for enzymatically conjugated antibodies were better than those of chemically modified analogues.