What question did this study set out to answer?

To determine how manipulating culture medium carbohydrate sources influences epithelial cell susceptibility and metabolic antioxidant defenses against nitric oxide-mediated oxidative stress.

December 15, 1999Biochemical JournalOpen Access

The in vitro manipulation of carbohydrate metabolism: a new strategy for deciphering the cellular defence mechanisms against nitric oxide attack

View Full Paper

Ask AI

Bookmark

Authors

CGClaire Le GoffeUniversity of Alabama at Birmingham GVG. ValletteCentre National de la Recherche Scientifique AJAnne JarryCentre National de la Recherche Scientifique

Discussion

Member takes

Implication

In vitro study reveals carbohydrate source manipulation alters nitric oxide sensitivity in colonic epithelial cells, indicating pentose phosphate pathway reducing power prevents injury.

Key Points

To determine how manipulating culture medium carbohydrate sources influences epithelial cell susceptibility and metabolic antioxidant defenses against nitric oxide-mediated oxidative stress.
Cultured polarized human colonic epithelial cells (HT29-Cl.16E) in media formulated with glucose, galactose, or galactose combined with 2-deoxyglucose.
Challenged cells with the nitric oxide donor PAPANONOate and evaluated pentose phosphate pathway utilization using 2-deoxy[1-(14)C]glucose radiolabeling to measure (14)CO(2) generation.
Epithelial cells exhibited severe cytotoxic and apoptotic cell death from nitric oxide exposure solely in galactose medium, which coincided with peroxynitrite generation, nitrotyrosine formation, and intracellular glutathione depletion.
Addition of non-glycolytic 2-deoxyglucose completely rescued galactose-fed cells from nitric oxide cytotoxicity and preserved glutathione reserves by routing through the pentose phosphate pathway.

Cite This Study

Goffe et al. (1999) studied this question.

synapsesocial.com/papers/6a7f2a7464b9ef61cb694a0e https://doi.org/10.1042/0264-6021:3440643

View Full Paper

Ask AI

Bookmark

Implication

In vitro study reveals carbohydrate source manipulation alters nitric oxide sensitivity in colonic epithelial cells, indicating pentose phosphate pathway reducing power prevents injury.

The in vitro manipulation of carbohydrate metabolism: a new strategy for deciphering the cellular defence mechanisms against nitric oxide attack

Discussion

Member takes

Implication

Key Points

Cite This Study

The in vitro manipulation of carbohydrate metabolism: a new strategy for deciphering the cellular defence mechanisms against nitric oxide attack

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider