What question did this study set out to answer?

Investigate the quaternary subunit structure of myosin by isolating and characterizing its alkali-dissociated heavy and light components.

September 1, 1966Proceedings of the National Academy of SciencesOpen Access

Subunit structure of myosin, II. Heavy and light alkali components.

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Authors

LGLewis C. GershmanAlbany Stratton VA Medical Center Albany PDPaul DreizenState University of New York ASA. StracherCornell University

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Implication

Biochemical analysis uncovers distinct heavy and light alkali subunits in myosin, highlighting the modular protein architecture driving muscle contraction.

Key Points

Investigate the quaternary subunit structure of myosin by isolating and characterizing its alkali-dissociated heavy and light components.
Dissociated purified myosin complexes under alkaline pH conditions to release constituent polypeptide chains.
Separated and analyzed the heavy and light alkali-stable protein fragments using biochemical fractionation techniques.
Demonstrated that alkaline treatment resolves intact myosin into separate heavy core chains and smaller light chain components.
Identified distinct structural properties and electrophoretic behavior between the dissociated heavy and light alkali subunits.

Cite This Study

Gershman et al. (1966) studied this question.

synapsesocial.com/papers/6a7f40435901e5fceb39d8cc https://doi.org/10.1073/pnas.56.3.966

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Implication

Biochemical analysis uncovers distinct heavy and light alkali subunits in myosin, highlighting the modular protein architecture driving muscle contraction.

Subunit structure of myosin, II. Heavy and light alkali components.

Discussion

Member takes

Implication

Key Points

Cite This Study

Subunit structure of myosin, II. Heavy and light alkali components.

Discussion

Member takes

Implication

Key Points

Cite This Study

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