Pitfalls in Measuring Cytokines

Letters15 July 1994Pitfalls in Measuring CytokinesSamir Kapadia, MD, Guillermo Torre-Amione, MD, PhD, and Douglas L. Mann, MDSamir Kapadia, MDSearch for more papers by this author, Guillermo Torre-Amione, MD, PhDSearch for more papers by this author, and Douglas L. Mann, MDSearch for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-121-2-199407150-00016 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:Casey and colleagues [1] note the potential importance of measuring cytokine levels in patients with systemic sepsis, whereas Dinarello and Cannon [2] detail many of the pitfalls associated with assaying cytokine levels. We report some other difficulties inherent in measuring “immunoreactive” tumor necrosis factor (TNF)- α by enzyme-linked immunosorbent assay (ELISA). As noted by Casey and colleagues [1], the assessment of TNF-α immunoreactivity reflects the level of the biologically active and inactive forms of TNF-α. However, the level of immunoreactive TNF-α is greatly influenced by the type of monoclonal antibody used in the ELISA kit, as well ...References1. Casey LC, Balk RA, Bone RC. Plasma cytokine and endotoxin levels correlate with survival in patients with the sepsis syndrome. Ann Intern Med. 1993; 119:771-8. Google Scholar2. Dinarello CA, Cannon JG. Cytokine measurements in septic shock. Ann Intern Med. 1993; 119:853-4. Google Scholar3. Van Zee KJ, Kohno T, Fischer E, Rock CS, Moldawer LL, Lowry SF. Tumor necrosis factor soluble receptors circulate during experimental and clinical inflammation and can protect against excessive tumor necrosis factor α in vitro and in vivo. Proc Natl Acad Sci U S A. 1992; 89:4845-9. Google Scholar Author, Article, and Disclosure InformationAuthors: Samir Kapadia, MD; Guillermo Torre-Amione, MD, PhD; Douglas L. Mann, MD PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byActivation of caspase-3 in the skeletal muscle during haemodialysisAge-stratification's role in cytokine based assay developmentCytokines and Myocardial Dysfunction: State of the ArtIntracellular Monocyte Cytokine Production and CD14 Expression Are Up-Regulated in Severe vs Mild Chronic Heart FailureChanges in Cytokine Production During Pregnancy in Patients with Graves' DiseasePlasma cytokines: what we are measuringRole of TNF in Mediating Renal Insufficiency Following Cardiac Surgery: Evidence of a Postbypass Cardiorenal SyndromeVariables That Affect Assays for Plasma Cytokines and Soluble Activation MarkersLevels of Cytokines and Immune Activation Markers in Plasma in Human Immunodeficiency Virus Infection: Quality Control ProceduresReference standards and quality performance considerations for measurements of cytokines and soluble markers of immune activationQuantification of cytokine mRNA in peripheral blood mononuclear cells using branched DNA (bDNA) technologyRole of Cytokines in the Mechanism of Action of Amlodipine: The PRAISE Heart Failure TrialTumor necrosis factor-? and tumor necrosis factor receptors in human heart failureCirculating Levels of Cytokines and Their Endogenous Modulators in Patients With Mild to Severe Congestive Heart Failure Due to Coronary Artery Disease or HypertensionProinflammatory cytokine levels in patients with depressed left ventricular ejection fraction: A report from the studies of left ventricular dysfunction (SOLVD)Variable estimates of cytokine levels produced by commercial ELISA kits: results using international cytokine standards 15 July 1994Volume 121, Issue 2Page: 149-150KeywordsCytokinesEnzyme linked immunosorbent assayForecastingMonoclonal antibodiesProteinsSepsisSerum proteinsShockT cellsTumor necrosis factor ePublished: 15 August 2000 Issue Published: 15 July 1994 Copyright & PermissionsCopyright © 1994 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...