IN STUDYING the nutritive value of poultry mashes with growing chicks the usual practice is to make up experimental pens of chicks without regard to sex. In fact unless crossbreeding is resorted to it is practically impossible to differentiate the sex of newly hatched chicks. Ordinarily as experiments progress it is found that the ratio of sexes in the various experimental pens is not uniform. Since the rate of growth of males and females is not identical, a question naturally arises as to whether the size and composition of the bones of the two sexes are identical. This question is not without significance since in many laboratories it is customary to judge concerning the extent of bone development by the ash content of tibiae removed from representative chicks at different stages of the experiment. Obviously, if the composition of chick tibiae varies with the sex then any variation in the . . .
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Holmes et al. (1932) studied this question.