Plasma Amino Acid Profile in Patients with Aortic Dissection

Figures

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  Figure 1. Overlaid chromatogram of the marker amino acids detected by multiple reaction monitoring (MRM).

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  Figure 2. The score plots of two-dimensional (A) and three-dimensional (B) PCA showed the changes in the human plasma amino acids data from CHD (●) and AD () groups (R2X = 68.7%, Q2 = 45.4%).

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  Figure 3. The score plots of two-dimensional (A) and three-dimensional (B) PCA showed the changes in the human plasma amino acids data from three groups. CHD group (●), Acute AD group (), Chronic AD group () (R2X = 72.0%, Q2 = 48.3%).

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  Figure 4. The score and loading plots of PLS-DA for the plasma samples in different groups. (A,B) PLS-DA score and loading plots of samples between CHD and Acute AD groups (R2X = 68.7%, Q2Y = 70.7%, Q2 = 42.9%); (C,D) PLS-DA score and loading plots of samples between CHD and Chronic AD groups (R2X = 46.0%, Q2Y = 89.2%, Q2 = 13.2%); (E,F) PLS-DA score and loading plots of samples between Acute AD and Chronic AD groups (R2X = 73.7%, Q2Y = 65.1%, Q2 = 39.6%). CHD group (●), Acute AD group (), Chronic AD group ).

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  Figure 5. Heat-map for key amino acids change color-scaled with z-scores for the human plasma where the warm (red) colored bars denote elevation of amino acids levels, and cold colored (blue) ones indicate a decrease compared to the CHD patients. Every amino acid is delegated by a single row of colored boxes, but columns correspond to different samples. Each value was standardized on the basis of the controls (A,B) CHD patients; (C) Chronic AD)) with subtracting mean and dividing by the standard deviation of controls (CHD patients). (A) Acute AD group versus CHD patients; (B) Chronic AD group versus CHD patients. (C) Acute AD group versus Chronic AD group.

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  Figure 6. The metabolic pathways of the most disregulated amino acids in plasma of AD patient. The tag in green represents the significant change of amino acids. The red boxes denote significant up-regulation of amino acids in Acute AD and Chronic AD groups compared with CHD group, while the blue boxes represent significant down-regulation. The black boxes denote no significant change.