What question did this study set out to answer?

To characterize full-length polycystin expression, tissue distribution, and subcellular localization to understand its normal physiological function and contribution to autosomal dominant polycystic kidney disease.

June 10, 1997Proceedings of the National Academy of SciencesOpen Access

Polycystin: In vitro synthesis, in vivo tissue expression, and subcellular localization identifies a large membrane-associated protein

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To characterize full-length polycystin expression, tissue distribution, and subcellular localization to understand its normal physiological function and contribution to autosomal dominant polycystic kidney disease.
Assembled authentic full-length PKD1 cDNA and carried out in vitro protein translation.
Generated polyclonal antibodies directed against specific extracellular and intracellular domains of polycystin for immunoprecipitation assays.
Evaluated tissue and subcellular localization via confocal microscopy and tissue section analyses across fetal, adult, and cystic renal cells, vascular endothelial cells, and extrarenal tissues.
Polycystin expressed predominantly in epithelial cells of the distal nephron and vascular endothelial cells in normal adult kidneys and maturing fetal nephrons, appearing in proximal nephrons only after injury-induced proliferation.
Extrarenal polycystin expression was restricted to ductal epithelium in the liver, pancreas, and breast, and to astrocytes in the brain.
Confocal microscopy revealed membrane localization of polycystin, specifically concentrating at lateral membranes upon cell-cell contact.

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Implication

In vitro and in vivo investigation demonstrates polycystin membrane localization in renal and epithelial tissues, suggesting vital roles in cell maturation and adhesion.

Cite This Study

Ibraghimov‐Beskrovnaya et al. (1997) studied this question.

synapsesocial.com/papers/6a7f92943e8043f4f642207f https://doi.org/10.1073/pnas.94.12.6397

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Implication

In vitro and in vivo investigation demonstrates polycystin membrane localization in renal and epithelial tissues, suggesting vital roles in cell maturation and adhesion.

Polycystin: In vitro synthesis, in vivo tissue expression, and subcellular localization identifies a large membrane-associated protein

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Discussion

Implication

Cite This Study

Polycystin: In vitro synthesis, in vivo tissue expression, and subcellular localization identifies a large membrane-associated protein

Key Points

Discussion

Implication

Cite This Study

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