March 1, 2000British Journal of PharmacologyOpen Access

Pharmacological characterization of small‐conductance Ca2+‐activated K+ channels stably expressed in HEK 293 cells

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Population

HEK 293 cells stably expressing hSK1 and rSK2 channels

Design

Preclinical

Authors

DSDorte StrøbækSaniona (Denmark)PCPalle ChristophersenSaniona (Denmark)PAPhilip K. AhringThe University of Sydney

Discussion

Member takes

Implication

Apamin/scyllatoxin show insufficient SK1/SK2 selectivity in mammalian cells; challenges hSK1 mediation of apamin-insensitive neuronal AHP and leaves mechanism open.

Structured PICO

Population

HEK 293 cells stably expressing hSK1 and rSK2 channels

Intervention

Pharmacological blockers including apamin, scyllatoxin, UCL 1684, dequalinium chloride, d-tubocurarine, and bicuculline methiodide

Outcome

IC50 values for channel blockade measured using whole-cell patch clamp recordingssurrogate

The pharmacological separation between SK channel subtypes expressed in mammalian cells is too small to support the notion that apamin-insensitive afterhyperpolarization of neurones is mediated by hSK1.

Cite This Study

Strøbæk et al. (2000) studied this question.

synapsesocial.com/papers/6a7f943d138fa0ec199fe839 https://doi.org/10.1038/sj.bjp.0703120

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Implication

Apamin/scyllatoxin show insufficient SK1/SK2 selectivity in mammalian cells; challenges hSK1 mediation of apamin-insensitive neuronal AHP and leaves mechanism open.

Pharmacological characterization of small‐conductance Ca2+‐activated K+ channels stably expressed in HEK 293 cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Pharmacological characterization of small‐conductance Ca2+‐activated K+ channels stably expressed in HEK 293 cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider