September 29, 2023Open Access

A simple and rapid preparation of smooth muscle myosin 2 for the electron microscopic analysis

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Why the study?

Current practices in myosin purification are time-consuming and cumbersome, and an increased actin to myosin ratio in smooth muscles adds complexity to the process.

Population

Actomyosin suspension of chicken gizzard tissues

Authors

ABAnahita Vispi BhardaKangwon National University HJHyun Suk JungKangwon National University

Discussion

Member takes

Implication

May accelerate structural myosin research; leaves open any cardiovascular clinical translation.

Structured PICO

Population

Actomyosin suspension from chicken gizzard tissues (2 grams)

Intervention

Phalloidin treatment followed by co-sedimentation and short column size exclusion chromatography

Comparator

Untreated actomyosin suspension (control)

Outcome

Purity and structural integrity of 10S myosin 2 molecules

A streamlined, phalloidin-based purification method allows for rapid isolation of highly pure and structurally intact smooth muscle myosin 2 for structural research.

Cite This Study

Bharda et al. (2023) studied this question.

synapsesocial.com/papers/6a7f9aa3fcb7d43d28406a5d https://doi.org/10.21203/rs.3.rs-3323619/v1

View Full Paper

Ask AI

Bookmark

A simple and rapid preparation of smooth muscle myosin 2 for the electron microscopic analysis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

A simple and rapid preparation of smooth muscle myosin 2 for the electron microscopic analysis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider