What question did this study set out to answer?

To develop a real-time single-cell imaging platform that overcomes bulk cell population averaging to capture the dynamic secretion of proteins alongside intracellular events.

April 22, 2014Scientific ReportsOpen Access

Real-time single-cell imaging of protein secretion

View Full Paper

Ask AI

Bookmark

Authors

YSYoshitaka ShirasakiTokyo University of Science MYMai YamagishiShanghai Cell Therapy Research Institute NSNobutake SuzukiShanghai Cell Therapy Research Institute

Discussion

Member takes

Implication

Experimental study demonstrates heterogeneous IL-1β secretion dynamics in single monocytes, highlighting real-time links between membrane integrity and cytokine release.

Key Points

To develop a real-time single-cell imaging platform that overcomes bulk cell population averaging to capture the dynamic secretion of proteins alongside intracellular events.
Fabricated sub-nanolitre-sized microwell arrays to isolate individual cells.
Applied a sandwich immunoassay monitored by total internal reflection fluorescence microscopy at 1-minute intervals to observe nonclassical IL-1β secretion in stimulated monocytes.
Detected significant heterogeneity in the onset time of IL-1β secretion among individual stimulated monocytes.
Identified a clear chronological relationship between the loss of cellular membrane integrity and the release of IL-1β during real-time live-cell monitoring.

Cite This Study

Shirasaki et al. (2014) studied this question.

synapsesocial.com/papers/6a7fa1d7a97e3300ffd76f18 https://doi.org/10.1038/srep04736

View Full Paper

Ask AI

Bookmark

Implication

Experimental study demonstrates heterogeneous IL-1β secretion dynamics in single monocytes, highlighting real-time links between membrane integrity and cytokine release.

Real-time single-cell imaging of protein secretion

Discussion

Member takes

Implication

Key Points

Cite This Study

Real-time single-cell imaging of protein secretion

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider