Why the study?
Cardiovascular diseases involve vascular smooth muscle cell and endothelial cell damage, but the effect of co-activating MasR and pGCA pathways in oxidative stress-induced VSMCs and ECs was unknown.
Does a synthesized novel peptide co-activating MasR and pGCA pathways protect primary vascular smooth muscle cells and endothelial cells against oxidative stress better than Ang1-7 or BNP alone?
Does a synthesized novel peptide co-activating MasR and pGCA pathways protect primary vascular smooth muscle cells and endothelial cells against oxidative stress better than Ang1-7 or BNP alone?
A novel peptide co-activating MasR and pGCA pathways provides superior protection against oxidative stress in vascular cells compared to BNP or Ang1-7 alone by inhibiting intracellular calcium.
Answers are grounded in this paper and cite their sources.
Should not yet change practice; leaves open whether dual-pathway peptides will protect human vessels from oxidative injury.
Cardiovascular diseases (CVDs) are associated with vascular smooth muscle cell (VSMC) and endothelial cell (EC) damage. Angiotensin1‐7 (Ang1‐7) and B‐type natriuretic peptide (BNP) are responsible for vasodilation and regulation of blood flow. These protective effects of BNP are primarily mediated by the activation of sGCs/cGMP/cGKI pathway. Conversely, Ang1‐7 inhibits Angiotensin II‐induced contraction and oxidative stress via Mas receptor activation. Thus, the aim of the study was to determine the effect of co‐activation of MasR and particulate guanylate cyclase receptor (pGCA) pathways by synthesized novel peptide (NP) in oxidative stress‐induced VSMCs and ECs. MTT and Griess reagent assay kits were used for the standardization of the oxidative stress (H 2 O 2 ) induced model in VSMCs. The expression of targeted receptors in VSMC was done by RT‐PCR and Western blot analysis. Protective effect of NP in VSMC and EC was determined by immunocytochemistry, FACS analysis, and Western blot analysis. Underlying mechanisms of EC‐dependent VSMC relaxation were done by determining downstream mRNA gene expression and intracellular calcium imaging of cells. Synthesized NP significantly improved oxidative stress‐induced injury in VSMCs. Remarkably, the actions of NP were superior to that of the Ang1‐7 and BNP alone. Further, a mechanistic study in VSMC and EC suggested the involvement of upstream mediators of calcium inhibition for the therapeutic effect. NP is reported to possess vascular protective activities and is also involved in the improvement of endothelial damage. Moreover, it is highly effective than that of individual peptides BNP and Ang1‐7 and therefore it may represent a promising strategy for CVDs.
No takes yet. Share an insight, caveat, or question.
Ghatage et al. (2023) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: