We demonstrate the cell-free expression of the actin-like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water-in-oil-in-water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.
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Martino et al. (2012) studied this question.
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