Beta-glucan promotes dental pulp healing by enhancing cell proliferation, migration, and mineralization

Background/purpose: Effective dental pulp healing is essential for preserving tooth vitality. Although beta-glucan has shown promise in wound healing in the medical fields, its potential effects on human dental pulp cells (HDPCs) remain unexplored. This study aimed to assess beta-glucan's effects on HDPC proliferation, migration, collagen synthesis, mineralization, and differentiation. Materials and methods: < 0.05. Results: At 24 and 72 h, all concentrations of beta-glucan significantly induced cell proliferation. In the wound healing assay, beta-glucan improved cell migration and increased the expression of integrin beta 1 after 24 h. Mineralized matrix formation and the expression of IL-10 and COL1 were significantly observed at 14 days. The upregulation of DSPP was detected in groups supplemented with 5 and 7.5 mg/mL beta-glucan. Conclusion: Beta-glucan enhanced cell proliferation, cell migration potential, integrin beta 1 expression, mineralized matrix formation, and DSPP, IL-10, and COL1 gene expression in HDPCs.