What question did this study set out to answer?

To determine how cyclin E interacts with the DNA replication factor MCM5 at centrosomes and whether this association controls centrosome duplication.

September 17, 2008Journal of Cell Science

Cyclin E-dependent localization of MCM5 regulates centrosome duplication

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Authors

RFRebecca FergusonUniversity of Colorado Anschutz Medical Campus JMJames L. MallerNorthwestern University

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Implication

Experimental study reveals that cyclin E targets MCM5 to centrosomes to inhibit over-duplication in CHO cells, suggesting DNA replication machinery coordinates centrosome division.

Key Points

To determine how cyclin E interacts with the DNA replication factor MCM5 at centrosomes and whether this association controls centrosome duplication.
Examined direct protein-protein interactions and centrosomal colocalization between cyclin E and MCM5, assessing dependence on the centrosomal localization sequence (CLS) and Cdk2 kinase activity.
Assayed centrosome over-duplication in S-phase-arrested Chinese hamster ovary (CHO) cells transfected with MCM5, its cyclin E-binding domain, or MCM2.
Cyclin E directly bound and colocalized with MCM5 at centrosomes via a CLS-dependent but Cdk2-independent mechanism through an evolutionarily conserved MCM5 domain.
Expression of full-length MCM5 or its isolated cyclin E-binding domain significantly inhibited centrosome over-duplication in S-phase-arrested CHO cells, whereas MCM2 expression had no inhibitory effect.

Cite This Study

Ferguson et al. (2008) studied this question.

synapsesocial.com/papers/6a7ff5bc2ea91e47663d324a https://doi.org/10.1242/jcs.034702

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Implication

Experimental study reveals that cyclin E targets MCM5 to centrosomes to inhibit over-duplication in CHO cells, suggesting DNA replication machinery coordinates centrosome division.

Cyclin E-dependent localization of MCM5 regulates centrosome duplication

Discussion

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Implication

Key Points

Cite This Study

Cyclin E-dependent localization of MCM5 regulates centrosome duplication

Discussion

Member takes

Implication

Key Points

Cite This Study

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