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To evaluate the methodological challenges of simulating cardiac ischemia-reperfusion injury in vitro and assess how calcium overload, reactive oxygen species generation, and pH shifts drive mitochondrial permeability transition.

March 18, 2015Frontiers in PhysiologyOpen Access

The calcium-ROS-pH triangle and mitochondrial permeability transition: challenges to mimic cardiac ischemia-reperfusion

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To evaluate the methodological challenges of simulating cardiac ischemia-reperfusion injury in vitro and assess how calcium overload, reactive oxygen species generation, and pH shifts drive mitochondrial permeability transition.
Critical commentary and theoretical analysis of experimental models used to simulate myocardial ischemia and reperfusion.
Evaluation of biochemical assays monitoring mitochondrial permeability transition pore opening in response to simultaneous shifts in calcium, reactive oxygen species, and intracellular pH.
Dynamic interactions among calcium accumulation, reactive oxygen species bursts, and rapid pH normalization act synergistically rather than as isolated triggers to open the mitochondrial permeability transition pore.
Conventional in vitro and ex vivo models frequently fail to replicate the precise temporal and spatial gradients of the calcium-ROS-pH triad observed during in vivo reperfusion.

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Commentary uncovers experimental challenges in modeling mitochondrial permeability transition during cardiac ischemia-reperfusion, highlighting interdependent calcium, reactive oxygen species, and...

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Cite This Study

Sabzali Javadov (2015) studied this question.

synapsesocial.com/papers/6a80010a04731064ad46f8ca https://doi.org/10.3389/fphys.2015.00083

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Implication

Commentary uncovers experimental challenges in modeling mitochondrial permeability transition during cardiac ischemia-reperfusion, highlighting interdependent calcium, reactive oxygen species, and...

The calcium-ROS-pH triangle and mitochondrial permeability transition: challenges to mimic cardiac ischemia-reperfusion

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Implication

Discussion

Cite This Study

The calcium-ROS-pH triangle and mitochondrial permeability transition: challenges to mimic cardiac ischemia-reperfusion

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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