Preclinical study demonstrates enhanced mRNA translation using glutathione-depleting nanoparticles in hard-to-transfect cells, highlighting a novel design principle for genetic therapies.
Effective messenger RNA (mRNA) transfection in hard-to-transfect cells delivered by vectors is a long-standing challenge. Now it is hypothesized that the high intracellular glutathione level is associated with suppressed mRNA translation. This theory leads to a new design principle of next-generation mRNA vectors: nanoparticles with glutathione depletion chemistry upregulate mRNA translation and enhance transfection, which is beneficial for mRNA delivery in hard-to-transfect cells in vitro and in vivo.
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Wang et al. (2019) studied this question.
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