What question did this study set out to answer?

To isolate and characterize the biochemical properties of a collagenase-active enzyme preparation from the salivary glands of the predatory mollusk Rapana venosa.

December 17, 2025The Ukrainian Biochemical JournalOpen Access

Isolation and characterization of collagenase-active preparation from Rapana venosa salivary glands

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Implication

Biochemical characterization reveals stable metallo- and cysteine collagenases in Rapana venosa salivary glands, highlighting a novel marine source for biomedical and industrial applications.

Key Points

To isolate and characterize the biochemical properties of a collagenase-active enzyme preparation from the salivary glands of the predatory mollusk Rapana venosa.
Extracted and purified salivary gland proteins using sequential acetone precipitation and ammonium sulfate fractionation.
Analyzed fractions via SDS-PAGE electrophoresis following the Laemmli protocol under reducing and non-reducing conditions.
Assayed proteolytic activity spectrophotometrically using collagen and gelatin substrates across varied pH levels, temperatures, and chemical inhibitor conditions.
Achieved a 13.8-fold purification across five protein fractions, with 22% of total proteins demonstrating collagenolytic activity and 88% showing gelatinolytic activity.
Identified bimodal pH optima at 4.5 and ~9.5, a thermal optimum at 46°C, and retention of approximately 90% enzyme activity over 8 hours at room temperature.
Determined the preparation is composed primarily of metalloproteinases and cysteine proteases based on total inhibition by metal chelators and activity enhancement by sulfhydryl reducing agents.

Cite This Study

A 2025 study studied this question.

synapsesocial.com/papers/6a802ceb1024bacc969291b2 https://doi.org/10.15407/ubj97.06.093

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Implication

Biochemical characterization reveals stable metallo- and cysteine collagenases in Rapana venosa salivary glands, highlighting a novel marine source for biomedical and industrial applications.

Isolation and characterization of collagenase-active preparation from Rapana venosa salivary glands

Discussion

Member takes

Implication

Key Points

Cite This Study

Isolation and characterization of collagenase-active preparation from Rapana venosa salivary glands

Discussion

Member takes

Implication

Key Points

Cite This Study

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