In a past few decades the variety of hemp Cannabis sativa L. was unfairly neglected because of the similarity with the kind of hemp that is illegal and is used as a narcotic. The objective of this study was to evaluate the oil extraction process from Cannabis sativa seeds by cold pressing followed by extraction with supercritical CO2. In pressing experiments, the response surface methodology was conducted in order to study the effects of temperature, frequency and nozzle size on oil recovery and quality parameters. The optimal condition to obtain the highest oil recovery and the best oil quality within the experimental range of the variables studied was at temperature of 60 °C, frequency of 20 Hz and using nozzle of ID 6 mm. The residual oil in the press cake was totally extracted by supercritical CO2 in newly designed and built supercritical fluid extraction system. The oregano essential oil was the most effective in protecting the oil from oxidative deterioration.
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Krunoslav Aladić (2015) studied this question.