Detection of cancer markers or marker surrogates by infrared spectral histopathology (SHP): PD-L1 and Her2/neu

In this contribution, data will be presented that suggest that certain cancer markers or their surrogates can be detected by infrared spectral imaging methodology. In particular, the co-localization of certain regions in spectral images with PD-L1-postive immunohistochemical response suggests that either the PD-L1 protein or a surrogate associated with its presence are detectable. In the case of Her2/neu, an analysis of mean spectral signatures of over forty thousand individual spectra revealed small, but statistically significant spectral differences between cancer marker positive and negative tissues in spectral regions associated with protein phosphorylation.