Why the study?
Does endotoxin-induced systemic inflammation increase shear stress-induced platelet plug formation measured by PFA-100 in healthy volunteers?
Does endotoxin-induced systemic inflammation increase shear stress-induced platelet plug formation measured by PFA-100 in healthy volunteers?
Systemic inflammation significantly alters PFA-100 results, which may confound the interpretation of platelet function tests in acute inflammatory states.
Acute inflammation alters PFA-100 results; confirms confounding of platelet function testing and warrants caution during acute illness.
The PFA‐100 measures platelet plug formation under shear stress and is strongly dependent on von Willebrand Factor (VWF) levels in plasma. We therefore hypothesized that elevated VWF levels, possibly as a result of acute inflammation, adversely influence PFA‐100 results. Healthy volunteers received either 2 ng/kg endotoxin or placebo in a randomized controlled trial. Four hours after endotoxin (but not placebo) infusion VWF levels increased by 85%, collagen epinephrine‐induced closure time (CT) decreased by 47% and collagen ADP‐CT decreased by 38% ( P < 0·0001) respectively. In conclusion, systemic inflammation has a major impact on the results obtained by PFA‐100 and may confound interpretation of platelet function.
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Homoncik et al. (2000) studied this question.
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