Analytical studies on the carbohydrates of grasses and clovers. IV.—further developments in the methods of estimation of mono‐, di‐ and oligo‐saccharides and fructosan

Methods for the determination of the water‐soluble carbohydrates in both fresh and ensiled materials are discussed. A technique has been developed for the estimation of the oligosaccharides in grasses, after their separation from the other free sugars by paper chromatography. Their fructose content is determined by a colorimetric method, and fructose, glucose and galactose are estimated after their complete hydrolysis with 0.5N‐sulphuric acid.