Existence and Functions of Two Enzymes with β‐Ketoadipate: Succinyl‐CoA Transferase Activity in Pseudomonas fluorescens

This work shows that Pseudomonas fluorescens can synthesize two different inducible enzymes which possess β‐ketoadipate: succinyl‐CoA transferase activity. Transferase I is operative in the activation of free β‐ketoadipic acid and is specifically induced in cells grown either with this acid, or with an aromatic precursor (benzoate) oxidized through the β‐ketoadipate pathway. Transferase II is specifically induced in cells grown with saturated dicarboxylic acids of chain length C 6 through C 10 , and its physiological function is that of an adipate: succinyl‐CoA transferase; its ability to activate β‐ketoadipic acid reflects non‐specificity with regard to the CoA acceptor.