First detection and complete genome sequence of a new potexvirus naturally infecting Adenium obesum

Figures

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  Fig. 1. (A) Adenium obesum leaves infected with AobVX and CMV showing mottling symptoms. (B) Morphology of AobVX virions, viewed by transmission electron microscopy. Samples were prepared as per Le Blanc et al. [5]. (C) Schematic representation of the genome organization of AobVX. The 5′ and 3′ UTRs are represented by solid horizontal bars, while the ORFs are depicted as open boxes. CP, coat protein; H, helicase domain; M, methyltransferase domain; R, RdRp domain; TGB, triple gene block; P1, TGB protein 1; P2, TGB protein 2; P3, TGB protein 3. (D) Aligment of small RNAs and Oxford Nanopore Technology (ONT) reads derived for sample MT483 onto the AobVX genome sequence. Scale bars on the y-axis show the total number of mapped reads (×1000).

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  Fig. 2. Neighbour-joining phylogenetic tree based on the predicted replicase amino acid sequence of AobVX and 63 members of the family Alphaflexiviridae, using 1000 bootstrap replicates. Analysis was performed using MEGA X. Percent boostrap values > 50 are shown at each major node.