Why the study?
Standardization of VIC calcification has not been implemented, impairing comparison across studies, and whether different culture methods impact the calcification phenotype of human VICs remained unknown.
Population
Primary human aortic VICs isolated from patients undergoing valve replacement
Comparison
Osteogenic media containing organic phosphate vs pro-calcifying media containing inorganic phosphate across cell passages
Design
In vitro experimental study
Authors
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Urges standardized pro-calcifying media for reproducible VIC studies; leaves open optimal in vitro models for calcific aortic valve disease.
Standardization of in vitro calcific aortic valve disease models is crucial, as human valvular interstitial cells exhibit passage-dependent and donor-dependent calcification in osteogenic media but not in pro-calcifying media.
Goto et al. (2019) studied this question.
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