Chromatography of the Coenzyme Q Family of Compounds on Silicone-impregnated Paper

SUMMARY mitochondria were incubated in air in the presence and absence Methods are described for the resolution of coenzyme Q of substrates, the ultraviolet absorption spectra of the cyclo- homologues and other lipides by means of reversed phase paper hexane extracts of such particles were shown to change in a chromatography on silicone-impregnat,ed filter paper. manner which denoted a substrate-dependent reduction of coenzyme Q (9). Such extracts have now been chromatographed Acknowledgments-The authors are indebted to Dr. David on paper, and the results have been consistent with the previous E. Green for his encouragement in the course of these studies. interpretation of the spectra of these extracts. The data given Oscar Mayer and Company kindly supplied the large quantity in Fig. 2 indicate that the extract of particles incubated in the of tissue used in the investigation. REFERENCES